The 3D Cardiac Strip assay is cultured in a 12-well screening format with three strips per well. Each strip contains around 250.000 hiPSC-cardiomyocytes on which we are able to reliably measure force of contraction, relaxation and contractile kinetics. This contractility assay is specifically developed for medium-throughput screening due to its low cell count. Our 3D Cardiac Strip is a first screening tool for target validation and drug development. Lead compounds can be followed-up in our Mini-Heart assay.